Herrmann H O, Hintze U, Gercken G
Mol Biochem Parasitol. 1981 Sep;3(5):319-25. doi: 10.1016/0166-6851(81)90005-0.
A medium-chain O-alkylglycerol, 1-O-[1'-14C]dodecyl-sn-glycerol, has been found to be incorporated into plasmenyl ethanolamine by Leishmania donovani promastigotes as revealed by radio gas-liquid chromatography; however, the ether bond of the administered O-alkyl-glycerol was cleaved extensively as judged from the occurrence of radioactive acyl moieties. The labelling pattern produced by the radioactive 'natural' 1-O-octadecyl-sn-glycerol was similar though the latter served as a slightly better substrate for plasmalogens. Experiments with the enantiomeric 3-O-alkyl-sn-glycerols in comparison revealed that these were poor substrates for plasmalogen synthesis, although they were taken up in identical amounts and cleaved even to a higher extent. Therefore, we conclude the 1-0-alkyl-sn-glycerols were utilized directly for plasmenyl ethanolamine synthesis. The incorporation of the dodecyl residue into plasmenyl ethanolamine did not affect the multiplication and shape of cells.
一种中链O-烷基甘油，即1-O-[1'-14C]十二烷基-sn-甘油，已被发现可被杜氏利什曼原虫前鞭毛体掺入缩醛磷脂乙醇胺中，这通过放射性气液色谱法得以揭示；然而，从放射性酰基部分的出现情况判断，所给予的O-烷基甘油的醚键被大量裂解。由放射性“天然”1-O-十八烷基-sn-甘油产生的标记模式相似，尽管后者作为缩醛磷脂的底物稍好一些。相比之下，对映体3-O-烷基-sn-甘油的实验表明，尽管它们的摄取量相同且裂解程度更高，但它们是缩醛磷脂合成的不良底物。因此，我们得出结论，1-O-烷基-sn-甘油直接用于缩醛磷脂乙醇胺的合成。十二烷基残基掺入缩醛磷脂乙醇胺中并不影响细胞的增殖和形态。
