Albouz S, Boutry J M, Dubois G, Bourdon R, Hauw J J, Baumann N
Naunyn Schmiedebergs Arch Pharmacol. 1981 Sep;317(2):173-7. doi: 10.1007/BF00500076.
To understand the mechanism of the lysosomal lipid storage induced by perhexiline maleate, we performed simultaneous lipid analysis and lysosomal enzymes determinations. Human fibroblasts were cultured for 5 days in the presence of perhexiline maleate at a concentration of 2 micrograms/ml of culture medium. Lipid analysis showed that those non toxic levels determined the same changes as seen with higher concentrations of the drug (Hauw et al. 1980) i. e. increase of cholesterol and of all major phospholipids. Qualitative phospholipid pattern was not markedly changed. Lysosomal enzymes activities were not modified with the exception of sphingomyelinase which was reduced to 12% of its normal level.
为了解马来酸哌克昔林诱导溶酶体脂质蓄积的机制，我们同时进行了脂质分析和溶酶体酶测定。将人成纤维细胞在含有浓度为2微克/毫升培养基的马来酸哌克昔林存在下培养5天。脂质分析表明，这些无毒水平产生的变化与较高浓度药物时所见相同（豪等人，1980年），即胆固醇和所有主要磷脂增加。磷脂定性模式没有明显改变。除鞘磷脂酶活性降至正常水平的12%外，溶酶体酶活性未发生改变。
