Foidart J, Foidart J, Dubois C, Mahieu P
Nephrologie. 1980;1(2):57-60.
The sera of 24 patients presenting with Good-pasture's syndrome, and of 30 normal blood donors were tested for the presence of anti-basement membrane (BM) antibodies, by a radioimmunological method, using two highly purified BM antigens, i.e. the type IV (BM) procollagen and a non-collagenous glycoprotein (laminin) isolated from the BM matrix secreted by a murine tumor. In marked contrast to the normal sera, the sera from 20 out of 24 patients presenting with Goodpasture's syndrome exhibited high anti-laminin and/or anti-type IV procollagen antibody levels. The other sera did not react with both BM antigens tested. The antibody titers varied according to the antigens used the sera tested. Kidney eluates from 4 "positive" patients also contained significant amounts of anti-laminin and anti-type IV procollagen antibodies. The data demonstrate that, in Goodpasture's syndrome, the pathogenicity of circulating and kidney-fixed anti-BM antibodies is not related to the chemical nature of the BM antigens triggering the immune response only. They suggest a polyclonal rather than a monoclonal stimulation in this disease.
采用放射免疫法，使用两种高度纯化的基底膜（BM）抗原，即IV型（BM）前胶原和从小鼠肿瘤分泌的BM基质中分离出的一种非胶原糖蛋白（层粘连蛋白），对24例患有Good-pasture综合征的患者血清以及30名正常献血者的血清进行检测，以确定抗基底膜抗体的存在。与正常血清形成显著对比的是，24例患有Goodpasture综合征的患者中，有20例患者的血清表现出高抗层粘连蛋白和/或抗IV型前胶原抗体水平。其他血清与所检测的两种BM抗原均无反应。抗体滴度因所用抗原和所检测血清而异。4例“阳性”患者的肾脏洗脱液中也含有大量的抗层粘连蛋白和抗IV型前胶原抗体。数据表明，在Goodpasture综合征中，循环和肾脏固定的抗BM抗体的致病性不仅仅与引发免疫反应的BM抗原的化学性质有关。它们提示在这种疾病中存在多克隆而非单克隆刺激。
