Okwuasaba F K, Weir B K, Cook D A, Krueger C A
Neurosurgery. 1981 Oct;9(4):402-6. doi: 10.1227/00006123-198110000-00010.
Samples of cerebrospinal fluid (CSF) drawn from patients with subarachnoid hemorrhage, subdural hemorrhage, or brain tumor, as well as control samples from patients without a known cerebral pathological condition, were tested for their ability to contract smooth muscle. Canine middle cerebral artery, canine basilar artery, and rat stomach fundus were used, and contractions were expressed as the percentage of the contraction elicited by a standard dose of 5-hydroxytryptamine. All samples that contained blood produced contractions of the smooth muscle preparations, and despite a large sample no significant differences were observed in the magnitude of the contractions either between preparations or between samples from different groups of patients. Control samples were generally without significant effect. Neither methysergide, a 5-hydroxytryptamine antagonist, nor indomethacin, an inhibitor of prostaglandin synthesis, significantly diminished the contractions induced by bloody CSF, although the calcium antagonist D600 successfully antagonized the response in all groups. D600 was a better antagonist of the action of blood-containing CSF on cerebral artery than on stomach fundus. Samples obtained from patients with angiographic evidence of vasospasm were significantly more active than those obtained from patients without vasospasm, but the latter retained considerable activity.
从蛛网膜下腔出血、硬膜下出血或脑肿瘤患者抽取的脑脊液（CSF）样本，以及来自无已知脑部病理状况患者的对照样本，均检测其收缩平滑肌的能力。使用犬大脑中动脉、犬基底动脉和大鼠胃底，收缩以标准剂量5-羟色胺引起的收缩百分比表示。所有含血样本均使平滑肌制剂产生收缩，尽管样本量很大，但在不同制剂之间或不同组患者的样本之间，收缩幅度均未观察到显著差异。对照样本通常无显著作用。5-羟色胺拮抗剂麦角新碱和前列腺素合成抑制剂吲哚美辛均未显著减弱血性脑脊液诱导的收缩，尽管钙拮抗剂D600成功拮抗了所有组的反应。D600对含血脑脊液对脑动脉的作用的拮抗作用比对胃底的作用更好。血管造影有血管痉挛证据的患者的样本比无血管痉挛患者的样本活性明显更高，但后者仍保留相当的活性。
