Hesse G, Schulze W, Wachtel E
Pharmazie. 1981 Sep;36(9):609-12.
It is dealt with modifications to the spectrophotometric determination of the alkylating behaviour of bifunctional nitrogen mustards with 4-(4'-nitrobenzyl)pyridine (NBP). The authors succeeded in characterizing spectrophotometrically, by means of a one-phase procedure, not only soluble, but also difficultly soluble, therapeutically known and newly synthetized mustards in regard to their alkylating behaviour. In the framework of in vitro studies, the authors report of experimental details of the proper preparation of biological material for the determination of residual activities, the analytical approach being always depending on the structure of the respective mustard. The differences in the time courses of the acylating attacks in serum, blood and suspensions of leukaemic cells are demonstrated by the example of some selected nitrogen mustards.
本文论述了对用4-(4'-硝基苄基)吡啶(NBP)分光光度法测定双功能氮芥烷基化行为的改进。作者成功地通过单相程序用分光光度法表征了不仅可溶的，而且难溶的、治疗上已知的和新合成的氮芥的烷基化行为。在体外研究的框架内，作者报告了用于测定残留活性的生物材料的适当制备的实验细节，分析方法总是取决于各自氮芥的结构。通过一些选定的氮芥的例子展示了血清、血液和白血病细胞悬液中酰化攻击时间进程的差异。
