Cohen M P, Wu V Y
Ren Physiol. 1981;4(2-3):112-20. doi: 10.1159/000172815.
In contrast to the fragmentation and loss of spatial arrangement seen in preparations of basement membrane isolated after sonic disruption, the architectural integrity of glomerular basement membrane is presented with the described technique of osmotic lysis and sequential detergent treatment. The biochemistry of components solubilized without pepsin digestion from GBM prepared with this technique is described.
与经声波破碎后分离的基底膜制剂中所见的空间排列碎片化和丧失形成对比的是，肾小球基底膜的结构完整性通过所描述的渗透裂解和连续去污剂处理技术得以呈现。本文描述了用该技术制备的未经胃蛋白酶消化而溶解的肾小球基底膜成分的生物化学特性。
