[甲基 - 3H]百草枯与大鼠、兔、仓鼠、小鼠和豚鼠肺蛋白的体外结合
Binding of [methyl-3H]paraquat to rat, rabbit, hamster, mouse and guinea pig lung proteins, in vitro.
作者信息
Giri S N, Lunsman P
出版信息
Toxicol Lett. 1981 Oct;9(2):93-100. doi: 10.1016/0378-4274(81)90023-0.
When incubated with the rat lung slices [methyl-3H]paraquat was found to bind covalently to acid-insoluble proteins. The evidence in the present study indicated that the binding of radioactivity was not due to the formation of a reactive metabolite secondary to mixed-function oxidase (MFO)-mediated bioactivation of paraquat but was probably due to formation of paraquat free radicals. Preincubation of lung slices with CN, 2,4-dinitrophenol (DPN), p-hydroxymercuribenzoate and cysteine inhibited binding, while anaerobic conditions has a marked stimulatory effect. There were no significant differences in the extent of covalent binding of [methyl-3H]paraquat to lung protein of rat. rabbit, mouse, guinea pig, and hamster.
当与大鼠肺切片一起孵育时，发现[甲基-³H]百草枯能与酸不溶性蛋白质共价结合。本研究中的证据表明，放射性的结合并非由于混合功能氧化酶（MFO）介导的百草枯生物活化产生的活性代谢产物的形成，而可能是由于百草枯自由基的形成。用氰化物、2,4-二硝基苯酚（DPN）、对羟基汞苯甲酸和半胱氨酸对肺切片进行预孵育可抑制结合，而厌氧条件则具有显著的刺激作用。[甲基-³H]百草枯与大鼠、兔子、小鼠、豚鼠和仓鼠的肺蛋白的共价结合程度没有显著差异。
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