Latif S A, McDermott M J, Morris D J
Steroids. 1981 Sep;38(3):307-19. doi: 10.1016/0039-128x(81)90066-0.
Among the tissues of the male rat studied, the largest quantities of the neutral polar metabolites of aldosterone were synthesized by the hepatic microsomal fraction. The polar metabolites of aldosterone were separated by HPLC into six peaks. Three peaks of non-polar (reduced) metabolites were also synthesized. Synthesis of at least four of the neutral polar metabolites was induced by phenobarbital and inhibited by both CO and SKF-525A. The rates of synthesis of these metabolites, which were linear up to 5 minutes, correlated well with the concentration of cytochrome P-450 in the liver microsomes. Addition of aldosterone to the microsomal fraction caused a pronounced type I change in the cytochrome P-450 spectrum. The half maximal spectral change (KS) for aldosterone was calculated to be 8 microM. These experiments indicate that the neutral polar metabolites of aldosterone are produced by cytochrome P-450 dependent hydroxy lations.
在所研究的雄性大鼠组织中，醛固酮的中性极性代谢产物大多由肝微粒体部分合成。醛固酮的极性代谢产物通过高效液相色谱法分离为六个峰。还合成了三个非极性（还原型）代谢产物峰。至少四种中性极性代谢产物的合成被苯巴比妥诱导，被一氧化碳和SKF - 525A抑制。这些代谢产物的合成速率在长达5分钟内呈线性，与肝微粒体中细胞色素P - 450的浓度密切相关。向微粒体部分添加醛固酮会导致细胞色素P - 450光谱出现明显的I型变化。醛固酮的半数最大光谱变化（KS）经计算为8微摩尔。这些实验表明，醛固酮的中性极性代谢产物是由细胞色素P - 450依赖性羟基化产生的。
