两种近交系小鼠美洲皮肤利什曼病和弥漫性皮肤利什曼病的比较研究
Comparative study of American cutaneous leishmaniasis and diffuse cutaneous leishmaniasis in two strains of inbred mice.
作者信息
Pérez H, Arredondo B, González M
出版信息
Infect Immun. 1978 Nov;22(2):301-7. doi: 10.1128/iai.22.2.301-307.1978.
Two Leishmania strains, AZV (isolated from a typical case of American cutaneous leishmaniasis) and AMP (from a case of diffuse cutaneous leishmaniasis), were studied in C57BL/6 and BALB/c mice. After infection with 10(4) amastigotes of either strain, C57BL/6 mice developed self-resolving lesions lasting 20 to 23 weeks and showed both delayed hypersensitivity response to leishmanial antigen and specific agglutinating antibodies. On the other hand, BALB/c mice infected with 10(4) AZV or AMP amastigotes developed chronic, large, ulcerated lesions and showed impaired cellular and humoral responses to the parasite. When BALB/c and C57BL/6 mice received 10(2) AMP amastigotes, patterns of infection were similar to those observed after inoculation of 10(4) amastigotes. In vitro studies revealed that spleen cells from AZV- or AMP-infected C57BL/6 mice showed an increased DNA-synthetic response to leishmanial antigen, concanavalin A, and phytohemagglutinin. Spleen cells from AZV- or AMP-infected BALB/c mice showed an increased response to concanavalin A and diminished responses to leishmanial antigen, phytohemagglutinin, and lipopolysaccharide.
在C57BL/6和BALB/c小鼠中研究了两种利什曼原虫菌株，AZV（从一例典型的美洲皮肤利什曼病病例中分离得到）和AMP（从一例弥漫性皮肤利什曼病病例中分离得到）。用10⁴个任一菌株的无鞭毛体感染后，C57BL/6小鼠出现持续20至23周的自愈性病变，并对利什曼原虫抗原表现出迟发型超敏反应以及特异性凝集抗体。另一方面，感染10⁴个AZV或AMP无鞭毛体的BALB/c小鼠出现慢性、大的溃疡性病变，并对该寄生虫的细胞和体液反应受损。当BALB/c和C57BL/6小鼠接种10²个AMP无鞭毛体时，感染模式与接种10⁴个无鞭毛体后观察到的相似。体外研究显示，来自感染AZV或AMP的C57BL/6小鼠的脾细胞对利什曼原虫抗原、刀豆球蛋白A和植物血凝素的DNA合成反应增强。来自感染AZV或AMP的BALB/c小鼠的脾细胞对刀豆球蛋白A的反应增强，而对利什曼原虫抗原、植物血凝素和脂多糖的反应减弱。
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