Werner J C, Whitman V, Fripp R R, Schuler H G, Morgan H E
Am J Physiol. 1981 Nov;241(5):E364-71. doi: 10.1152/ajpendo.1981.241.5.E364.
Metabolic activity of the newborn pig heart was observed by means of a preparation that allowed isolated hearts to perform a physiologic work load. A 10-min period of retrograde aortic perfusion was followed by 60 min of recirculating perfusion in which bicarbonate buffer was infused into the right atrium and ejected from both ventricles at measured pressures and flow rates. The buffer was supplemented with either glucose (10 mM), lactate (20 mM), or pyruvate (20 mM), or was unsupplemented. Pressure development and ventricular outputs were stable through 60 min of perfusion with glucose, pyruvate, or lactate supplementation. Myocardial high-energy phosphate and glycogen contents were also stable. In hearts perfused without substrate supplementation, mechanical performance deteriorated after 30 min. Rates of substrate uptake and oxygen consumption indicated that either glucose, lactate, or pyruvate could serve as a primary source of metabolic energy. Minimal lactate production was observed when either glucose or pyruvate were present. These findings indicate that, despite recent emergence from the relatively hypoxic intrauterine environment, the well-oxygenated newborn pig heart did not depend on anaerobic metabolism of glucose for energy production at physiologic work loads.
通过一种能使离体心脏承受生理工作负荷的制备方法，观察新生猪心脏的代谢活性。先进行10分钟的逆行主动脉灌注，随后进行60分钟的循环灌注，在此过程中，将碳酸氢盐缓冲液注入右心房，并在设定压力和流速下从两个心室排出。缓冲液中添加了葡萄糖（10 mM）、乳酸盐（20 mM）或丙酮酸盐（20 mM），或者不添加任何物质。在补充葡萄糖、丙酮酸盐或乳酸盐的情况下，灌注60分钟期间压力的产生和心室输出保持稳定。心肌高能磷酸盐和糖原含量也保持稳定。在未补充底物的情况下灌注的心脏，30分钟后机械性能恶化。底物摄取率和耗氧率表明，葡萄糖、乳酸盐或丙酮酸盐均可作为代谢能量的主要来源。当存在葡萄糖或丙酮酸盐时，观察到乳酸产生量极少。这些发现表明，尽管新生猪刚刚从相对缺氧的子宫内环境中娩出，但在生理工作负荷下，充分氧合的新生猪心脏并不依赖葡萄糖的无氧代谢来产生能量。
