Klunker R, Kiesow I
Angew Parasitol. 1981 Aug;22(3):131-43.
The suitability of in vitro-feeding for the laboratory rearing of Ornithodoros moubata Murray was tested by the development of about 3,000 first-stage nymphs to the adult stage. At intervals of 3 to 4 weeks defibrinated blood of sheep was fed to the ticks through a parafilm "M" membrane on an electric hot plate. On average 61% of the ticks were found engorged after the feeding. The highest rate of about 78% in the first nymphal stage decreased to about 45% in the fifth. The increase was highest in the second and third stages and lowest in the fifth. The total survival rate in this experiment was about 46%. Losses by the feeding could be reduced by means of some improvements in the experiment.
通过大约3000只第一期若虫发育至成虫阶段,测试了体外喂养用于莫氏钝缘蜱实验室饲养的适用性。每隔3至4周,将羊的去纤维蛋白血通过“M”型保鲜膜在电热板上喂给蜱虫。喂食后平均发现61%的蜱虫饱食。若虫第一阶段约78%的最高饱食率在第五阶段降至约45%。饱食率的增加在第二和第三阶段最高,在第五阶段最低。本实验中的总存活率约为46%。通过实验中的一些改进可以减少喂食造成的损失。