Uckert W, Wunderlich V, Sydow G, Niezabitowski A
Arch Geschwulstforsch. 1981;51(5):386-93.
The production of a type D retrovirus (PMFV) was analysed using different conditions of cultivation. Both in cell culture flasks and in roller culture bottles with discontinuous change of medium an increase of virus amount was determined within 12 h after seeding of virus-infected cells. Then the virus production considerably fluctuated around an apparent mean value until the end of the experiment at 56 h. In case of a continuous change of cell culture medium in roller bottles a maximum of virus production was measured at a retention time of 24 h of cell culture medium. The production of PMFV could be essentially increased when virus-producing cells were co-cultivated with non-infected cells of the same type.
使用不同的培养条件分析了D型逆转录病毒（PMFV）的产生情况。在细胞培养瓶以及培养基不连续更换的转瓶培养中，在接种病毒感染细胞后的12小时内可检测到病毒量增加。然后，直到实验结束的56小时，病毒产生量在一个明显的平均值附近大幅波动。在转瓶中连续更换细胞培养基的情况下，当细胞培养基保留时间为24小时时可测得最大病毒产量。当产病毒细胞与相同类型的未感染细胞共培养时，PMFV的产量可显著增加。
