用于精确二维聚丙烯酰胺凝胶电泳分析的组织蛋白双标记还原甲基化
Double-label reductive methylation of tissue proteins for precision two-dimensional polyacrylamide-gel electrophoretic analysis.
作者信息
Finger J M, Choo K H
出版信息
Biochem J. 1981 Jan 1;193(1):371-4. doi: 10.1042/bj1930371.
Abstract
Reductive methylation has been used to radioactively label crude-extract proteins with 3H or 14C. The procedure achieved good isotope incorporation and resolution of proteins on two-dimensional polyacrylamide gels. It allows high-precision comparison of tissue samples by double-labelling and should facilitate the study of tissue proteins by two-dimensional gel electrophoresis.
摘要
还原甲基化已被用于用³H或¹⁴C对粗提物蛋白质进行放射性标记。该方法在二维聚丙烯酰胺凝胶上实现了良好的同位素掺入和蛋白质分辨率。它允许通过双标记对组织样本进行高精度比较，并应有助于通过二维凝胶电泳研究组织蛋白质。
Zotero 插件