新生物合成的细胞色素P-450与用苯巴比妥诱导的大鼠肝脏的高尔基体相关。
Newly biosynthesized cytochrome P-450 associated with the golgi apparatus from livers of rats induced with phenobarbital.
作者信息
Cooper M B, Craft J A, Rees D E, Rabin B R
出版信息
Biochem J. 1981 Mar 15;194(3):691-7. doi: 10.1042/bj1940691.
Abstract
- Cytochrome P-450 has been detected in preparations of golgi apparatus from the livers of phenobarbital-induced rats. 2. Newly biosynthesized cytochrome P-450 is also present in preparations of golgi apparatus. By using three different techniques to fractionate the golgi into vesicle contents and membrane components it was found that newly biosynthesized cytochrome P-450 is associated solely with the membrane fraction. 3. By investigating the susceptibility of cytochrome P-450, present in the golgi apparatus, to the action of trypsin it was found that the majority was oriented on the cytosolic face of the membrane.
摘要
- 在苯巴比妥诱导的大鼠肝脏高尔基体的制备物中检测到了细胞色素P-450。2. 新生物合成的细胞色素P-450也存在于高尔基体的制备物中。通过使用三种不同的技术将高尔基体分离为囊泡内容物和膜成分，发现新生物合成的细胞色素P-450仅与膜部分相关。3. 通过研究高尔基体中存在的细胞色素P-450对胰蛋白酶作用的敏感性，发现大多数细胞色素P-450位于膜的胞质面上。
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