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新生物合成的细胞色素P-450与用苯巴比妥诱导的大鼠肝脏的高尔基体相关。

Newly biosynthesized cytochrome P-450 associated with the golgi apparatus from livers of rats induced with phenobarbital.

作者信息

Cooper M B, Craft J A, Rees D E, Rabin B R

出版信息

Biochem J. 1981 Mar 15;194(3):691-7. doi: 10.1042/bj1940691.

DOI:10.1042/bj1940691
PMID:7306018
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1162803/
Abstract
  1. Cytochrome P-450 has been detected in preparations of golgi apparatus from the livers of phenobarbital-induced rats. 2. Newly biosynthesized cytochrome P-450 is also present in preparations of golgi apparatus. By using three different techniques to fractionate the golgi into vesicle contents and membrane components it was found that newly biosynthesized cytochrome P-450 is associated solely with the membrane fraction. 3. By investigating the susceptibility of cytochrome P-450, present in the golgi apparatus, to the action of trypsin it was found that the majority was oriented on the cytosolic face of the membrane.
摘要
  1. 在苯巴比妥诱导的大鼠肝脏高尔基体的制备物中检测到了细胞色素P-450。2. 新生物合成的细胞色素P-450也存在于高尔基体的制备物中。通过使用三种不同的技术将高尔基体分离为囊泡内容物和膜成分，发现新生物合成的细胞色素P-450仅与膜部分相关。3. 通过研究高尔基体中存在的细胞色素P-450对胰蛋白酶作用的敏感性，发现大多数细胞色素P-450位于膜的胞质面上。
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db6a/1162803/d835e64933b8/biochemj00403-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db6a/1162803/31917a87b241/biochemj00403-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db6a/1162803/d835e64933b8/biochemj00403-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db6a/1162803/31917a87b241/biochemj00403-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db6a/1162803/d835e64933b8/biochemj00403-0048-a.jpg

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1
Newly biosynthesized cytochrome P-450 associated with the golgi apparatus from livers of rats induced with phenobarbital.新生物合成的细胞色素P-450与用苯巴比妥诱导的大鼠肝脏的高尔基体相关。
Biochem J. 1981 Mar 15;194(3):691-7. doi: 10.1042/bj1940691.
2
Differential time-course of induction of rat liver gamma-glutamyltransferase and drug-metabolizing enzymes in the endoplasmic reticulum, Golgi and plasma membranes after a single phenobarbital injection. Evaluation of protein variations by two-dimensional electrophoresis.单次注射苯巴比妥后，大鼠肝脏内质网、高尔基体和质膜中γ-谷氨酰转移酶及药物代谢酶诱导的差异时程。通过二维电泳评估蛋白质变化。
Cell Biochem Funct. 1987 Jul;5(3):217-31. doi: 10.1002/cbf.290050309.
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Induction of cytochrome P450 by phenobarbital in rat liver visualized by monoclonal antibody immunoelectron microscopy in situ.用单克隆抗体免疫电子显微镜原位观察苯巴比妥对大鼠肝脏细胞色素P450的诱导作用。
Eur J Cell Biol. 1990 Aug;52(2):193-200.
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Induction by phenobarbital of the mRNA for a specific variant of rat liver microsomal cytochrome P-450.苯巴比妥诱导大鼠肝微粒体细胞色素P - 450特定变体的mRNA产生。
Biochem J. 1981 Jun 15;196(3):839-51. doi: 10.1042/bj1960839.
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[Induction of the phenobarbital form of cytochrome P-450 by chemically unrelated compounds].
Biokhimiia. 1986 Jul;51(7):1223-9.
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Formation of cytochrome P-450 containing haem or cobalt-protoporphyrin in liver homogenates of rats treated with phenobarbital and allylisopropylacetamide.用苯巴比妥和烯丙异丙基乙酰胺处理的大鼠肝脏匀浆中含血红素或钴原卟啉的细胞色素P - 450的形成。
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Effect of thioacetamide on cytochrome P-450 synthesis in rat liver.硫代乙酰胺对大鼠肝脏细胞色素P - 450合成的影响。
Biochem J. 1984 Mar 1;218(2):371-7. doi: 10.1042/bj2180371.
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[Immunomorphological study of cytochrome P-450PB induction in the liver of rat embryos and pregnant females after phenobarbital administration].
Biull Eksp Biol Med. 1985 Mar;99(3):366-8.
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Site of biosynthesis of cytochrome P450 in hepatocytes of phenobarbital treated rats.
Biochem Biophys Res Commun. 1976 Aug 23;71(4):1153-60. doi: 10.1016/0006-291x(76)90774-9.
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Isolation of Golgi fractions from colchicine-treated rat liver. II. Electrophoretic characterization.从秋水仙碱处理的大鼠肝脏中分离高尔基体组分。II. 电泳特性分析。
Biochim Biophys Acta. 1979 Apr 4;552(2):225-37. doi: 10.1016/0005-2736(79)90279-7.

引用本文的文献

1
Cytochrome P450: a novel system modulating Ca2+ channels and contraction in mammalian heart cells.细胞色素P450：调节哺乳动物心脏细胞中钙离子通道和收缩的新系统。
J Physiol. 1998 May 1;508 ( Pt 3)(Pt 3):777-92. doi: 10.1111/j.1469-7793.1998.777bp.x.

本文引用的文献

1
The role of organelles in the chemical modification of the primary translation products of secretory proteins.细胞器在分泌蛋白初级翻译产物化学修饰中的作用。
FEBS Lett. 1976 Dec 31;72(2):215-26. doi: 10.1016/0014-5793(76)80973-8.
2
THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. II. SOLUBILIZATION, PURIFICATION, AND PROPERTIES.肝微粒体的一氧化碳结合色素。II. 增溶、纯化及性质
J Biol Chem. 1964 Jul;239:2379-85.
3
Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase.
营养不良性肌肉的生物化学。线粒体琥珀酸 - 四氮唑还原酶和三磷酸腺苷酶。
Biochem J. 1961 Sep;80(3):649-54. doi: 10.1042/bj0800649.
4
Asymmetric distribution of cytochrome P-450 and NADPH--cytochrome P-450 (cytochrome c) reductase in vesicles from smooth endoplasmic reticulum of rat liver.细胞色素P - 450和NADPH - 细胞色素P - 450（细胞色素c）还原酶在大鼠肝脏滑面内质网囊泡中的不对称分布。
Biochem J. 1980 Sep 15;190(3):737-46. doi: 10.1042/bj1900737.
5
Gluconeogenesis in the perfused rat liver.灌注大鼠肝脏中的糖异生作用。
Biochem J. 1966 Nov;101(2):284-92. doi: 10.1042/bj1010284.
6
The estimation of ribonucleic acid using ultraviolet absorption measurements.利用紫外线吸收测量法对核糖核酸进行估算。
Biochim Biophys Acta. 1965 Nov 8;108(3):333-9. doi: 10.1016/0005-2787(65)90025-0.
7
Cytochrome b5 and CO-binding cytochromes in the Golgi membranes of mammalian livers.哺乳动物肝脏高尔基体膜中的细胞色素b5和一氧化碳结合细胞色素
Biochem Biophys Res Commun. 1970 Jul 27;40(2):297-305. doi: 10.1016/0006-291x(70)91009-0.
8
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
9
The effects of aflatoxin B1 on the sex-specific binding of steroid hormones to microsomal membranes of rat liver.黄曲霉毒素B1对大鼠肝脏微粒体膜上甾体激素性别特异性结合的影响。
Eur J Biochem. 1971 Jun 29;20(4):580-6. doi: 10.1111/j.1432-1033.1971.tb01430.x.
10
Isolation of a Golgi apparatus-rich fraction from rat liver. I. Method and morphology.从大鼠肝脏中分离富含高尔基体的组分。I. 方法与形态学。
J Cell Biol. 1970 Mar;44(3):484-91. doi: 10.1083/jcb.44.3.484.