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来自颗粒海胆精子的组蛋白H1的制备与表征

Preparation and characterization of histone H1 from the sperm of the sea-urchin Sphaerechinus granularis.

作者信息

Giancotti V, Cosimi S, Cary P D, Crane-Robinson C, Geraci G

出版信息

Biochem J. 1981 Apr 1;195(1):171-6. doi: 10.1042/bj1950171.

Abstract

The separation and purification of histone H1 from the sperm of the sea-urchin Sphaerechinus granularis is described. Physical studies were used to compare this histone H1 molecule with H1 histones from other species. C.d. and 270 MHz n.m.r. spectroscopy indicate that, despite significant compositional differences from other sea-urchin sperm H1 histones, their secondary and tertiary structures are very similar. A large difference in helicity was, however, found between S. granularis histone H1 and calf thymus histone H1, and their n.m.r. and fluorescence spectra also differ considerably. It is concluded that secondary structure and tertiary structure have not been conserved in the evolution of the H1 histone family.

摘要

本文描述了从粒刺球海胆精子中分离和纯化组蛋白H1的过程。通过物理研究将这种组蛋白H1分子与其他物种的H1组蛋白进行了比较。圆二色光谱和270兆赫核磁共振光谱表明,尽管与其他海胆精子H1组蛋白在组成上存在显著差异,但其二级和三级结构非常相似。然而,发现粒刺球海胆组蛋白H1与小牛胸腺组蛋白H1在螺旋度上有很大差异,它们的核磁共振光谱和荧光光谱也有很大不同。得出的结论是,H1组蛋白家族在进化过程中二级结构和三级结构并未保守。

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The role of histones in the maintenance of chromatin structure.组蛋白在维持染色质结构中的作用。
Proc Natl Acad Sci U S A. 1965 Oct;54(4):1204-12. doi: 10.1073/pnas.54.4.1204.
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Chromosome structure.染色体结构
Annu Rev Genet. 1970;4:263-94. doi: 10.1146/annurev.ge.04.120170.001403.
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Biochim Biophys Acta. 1974 Aug 8;359(2):341-5. doi: 10.1016/0005-2795(74)90233-5.

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