海胆精子H2B组蛋白的物种特异性和个体变异性。

Species specificity and individual variability of sea urchin sperm H2B histones.

作者信息

de Petrocellis B, de Petrocellis L, Lancieri M, Geraci G

出版信息

Cell Differ. 1980;9(4):195-202. doi: 10.1016/0045-6039(80)90018-4.

Abstract

Total histones from the sperms and embryos of the sea urchins Paracentrotus lividus, Arbacia lixula, Psammechinus microtuberculatus and Sphaerechinus granularis hae been fractionated into the component molecules by electrophoretic analyses in SDS, in urea-acetic acid and in Triton-urea-acetic acid. Sperm H2B histones are in all cases different from those of the corresponding embryonic chromatins. Each sea urchin species has distinctive variants of the sperm H2B histones that are fractionated by electrophoresis in SDS acrylamide gel into two to four components forming a new class of lower mobility. This analytical method shows that individuals of the same species have different assortments of the H2B components. Electrophoretic analyses in Triton-urea also show multiple components for H2B but the patterns are similar in the different individuals.

摘要

通过在十二烷基硫酸钠（SDS）、尿素 - 乙酸以及 Triton - 尿素 - 乙酸体系中进行电泳分析，将地中海海胆、紫球海胆、微小海胆和粒球海胆精子与胚胎中的总组蛋白分离成各个组成分子。在所有情况下，精子 H2B 组蛋白均与相应胚胎染色质中的 H2B 组蛋白不同。每种海胆都有独特的精子 H2B 组蛋白变体，这些变体在 SDS 聚丙烯酰胺凝胶中通过电泳分离为两到四个组分，形成一类新的低迁移率组分。这种分析方法表明，同一物种的个体具有不同的 H2B 组分组合。在 Triton - 尿素体系中的电泳分析也显示 H2B 有多个组分，但不同个体中的图谱相似。