芥菜（Brassica campestris）线粒体中含铁超氧化物歧化酶的缺失。
Absence of the iron-containing superoxide dismutase in mitochondria from mustard (Brassica campestris).
作者信息
Salin M L, Bridges S M
出版信息
Biochem J. 1981 Apr 1;195(1):229-33. doi: 10.1042/bj1950229.
Abstract
Mitochondria were isolated from mature leaves as well as etiolated seedlings of Brassica campestris (mustard), a eukaryote previously shown to possess the iron-containing isoenzyme of superoxide dismutase. On the basis of KCN- and H2O2-sensitivity, and on polyacrylamide-gel analysis, only the cuprozinc and mangano superoxide dismutases were found in mitochondria. The iron-containing enzyme was absent. The mangano enzyme was found in the mitochondrial matrix, whereas the cuprozinc enzyme appeared to be localized in the intermembrane space.
摘要
从白菜（芥菜）的成熟叶片以及黄化幼苗中分离出线粒体，白菜是一种先前已被证明含有含铁超氧化物歧化酶同工酶的真核生物。基于对氰化钾和过氧化氢的敏感性以及聚丙烯酰胺凝胶分析，在线粒体中仅发现了铜锌超氧化物歧化酶和锰超氧化物歧化酶。未发现含铁酶。锰酶存在于线粒体基质中，而铜锌酶似乎定位于膜间隙。
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