苦瓜凝集素与配体结合的荧光研究。
Fluorimetric studies of the binding of Momordica charantia (bitter gourd) lectin with ligands.
作者信息
Das M K, Khan M I, Surolia A
出版信息
Biochem J. 1981 Apr 1;195(1):341-3. doi: 10.1042/bj1950341.
Abstract
The association constants for the binding of a series of ligands with a galactose-specific lectin from Momordica charantia (bitter gourd) has been determined through the ligand-induced quenching of protein fluorescence. Analysis of the iodide quenching suggested that there is a slight increase in the accessibility of tryptophan residues of the lectin on binding lactose.
摘要
通过配体诱导的蛋白质荧光猝灭，已测定了一系列配体与来自苦瓜的半乳糖特异性凝集素的结合常数。碘化物猝灭分析表明，凝集素与乳糖结合时色氨酸残基的可及性略有增加。
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Biochemistry. 1971 Aug 17;10(17):3254-63. doi: 10.1021/bi00793a015.
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