[两种烟草在液体培养中的染色体变异性]
[Chromosomal variability of 2 species of Nicotiana in fluid culture].
作者信息
Balzan S, Ronchi V N
出版信息
Boll Soc Ital Biol Sper. 1981 Aug 15;57(15):1596-600.
The chromosomal number in relation with the age of suspension culture of nicotiana glauca (NG) and the non tumorous N. glauca x N. langsdorffii hybrid has been studied. In both species, an higher variability of chromosome number is present in younger culture. Aged cultures show a stabilization, for NG, around the hexaploid number (3n = 72) and for NNT, which is an amphidiploid specie, on the tetraploid level (4n = 84). Both species show, with the age of culture, a decrease in plant regeneration capacity, which is not due to chromosomal variability, since young cultures are much more variable than older one.
研究了与烟草（NG）悬浮培养年龄以及非肿瘤性烟草×郎氏烟草杂种相关的染色体数目。在这两个物种中，较年轻的培养物中染色体数目的变异性更高。老化的培养物表现出稳定状态，对于烟草而言，稳定在六倍体数目（3n = 72）左右，对于作为双二倍体物种的非肿瘤性烟草×郎氏烟草杂种，稳定在四倍体水平（4n = 84）。随着培养年龄的增长，这两个物种的植株再生能力均下降，这并非由于染色体变异性所致，因为年轻培养物的变异性比老化培养物大得多。
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