Prosser J, Reisner A H, Bradley M L, Ho K, Vincent P C
Biochim Biophys Acta. 1981 Nov 27;656(1):93-102. doi: 10.1016/0005-2787(81)90031-9.
Total human DNA was fractionated from the three types of Cs2SO4 gradient used to prepare satellites I, II and III. Three satellite DNAs were found: satellite I with a mean buoyant density of 1.6888 g/ml comprising about 1.3% of the total, satellite II with a mean buoyant of 1.696 g/ml, comprising about 1% of the total an satellite III with a mean buoyant density of 1.699 g/ml comprising about 2.2% of the total. The buoyant densities of these satellites after purification were 1.686, 1.694 and 1.697 m/gl, respectively. A preparation with the attributes of satellite IV was isolated from the shoulder region of a satellite III preparative gradient. In situ hybridization using complementary RNA showed that the three satellites were located predominantly on chromosomes 9, Y, 15 and 1. Satellite II also showed marked hybridization to chromosome 16. Satellites I and II and III cross-hybridized to each other but satellites I and II did not. On the basis of our hybridization data, we suggest that some of the same sequences which comprise satellite III also comprise satellite I an II.
从用于制备卫星DNA I、II和III的三种硫酸铯梯度类型中分离出了人类总DNA。发现了三种卫星DNA：卫星I的平均浮力密度为1.6888 g/ml，约占总量的1.3%；卫星II的平均浮力密度为1.696 g/ml，约占总量的1%；卫星III的平均浮力密度为1.699 g/ml，约占总量的2.2%。纯化后这些卫星DNA的浮力密度分别为1.686、1.694和1.697 m/gl。从卫星III制备梯度的肩部区域分离出了具有卫星IV特征的制剂。使用互补RNA进行的原位杂交表明，这三种卫星主要位于9号、Y、15号和1号染色体上。卫星II与16号染色体也有明显的杂交信号。卫星I、II和III彼此之间存在交叉杂交，但卫星I和II之间没有。根据我们的杂交数据，我们认为构成卫星III的一些相同序列也构成了卫星I和II。
