人类主带DNA中的重复核苷酸序列。

Repeated nucleotide sequences in human main band DNA.

作者信息

Corneo G, Nelli L C, Meazza D, Ginelli E

出版信息

Biochim Biophys Acta. 1980 May 30;607(3):438-44. doi: 10.1016/0005-2787(80)90154-9.

Abstract

Male and female human placenta DNA was fractionated in an Ag+-Cs2SO4 density gradient. The different fractions along the gradient were analyzed by Hae III endonuclease digestion. Within the main band DNA on the light side a component having a Hae III digestion pattern similar to that of human satellite III DNA has been identified. This component which might be defined as a cryptic satellite accounts for at least 3% of the total human DNA and has a different position than human satellite III in Ag+-Cs2SO4.

摘要

将男性和女性的人胎盘DNA在Ag⁺-Cs₂SO₄密度梯度中进行分级分离。沿着梯度的不同级分通过Hae III核酸内切酶消化进行分析。在轻侧的主带DNA内，已鉴定出一种具有与人卫星III DNA相似的Hae III消化模式的成分。这种可能被定义为隐蔽卫星的成分至少占人类总DNA的3%，并且在Ag⁺-Cs₂SO₄中与人卫星III的位置不同。