一种用于胆固醇检测的偶联烟酰胺腺嘌呤二核苷酸过氧化物酶分光光度法。
A coupled NAD+-peroxidase spectrophotometric assay for cholesterol.
作者信息
Avigad G, Robertson B
出版信息
Clin Chem. 1981 Dec;27(12):2035-7.
PMID:7307256
Abstract
We describe a modified enzymic reagent for determination of cholesterol and cholesterol ester in serum and in high-density lipoprotein. In this new procedure, the hydrogen peroxide produced by the action of cholesterol oxidase is used as a substrate for NAD+ peroxidase. Spectrophotometric determination of the NADH consumed in this coupled reaction provides a direct and absolute measure of the cholesterol originally present in free form and that liberated by the action of cholesterol ester hydrolase.
摘要
我们描述了一种用于测定血清和高密度脂蛋白中胆固醇及胆固醇酯的改良酶试剂。在这一新方法中，胆固醇氧化酶作用产生的过氧化氢被用作NAD⁺过氧化物酶的底物。对该偶联反应中消耗的NADH进行分光光度测定，可直接且绝对地测量最初以游离形式存在的胆固醇以及由胆固醇酯水解酶作用释放出的胆固醇。
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