Thapliyal R C, Maddocks J L
J Chromatogr. 1978 Oct 11;160(1):239-45. doi: 10.1016/s0021-9673(00)91801-6.
A method is described for converting thiol metabolites of azathioprine and 6-mercaptopurine into phenyl mercury derivatives. Separation of these derivatives was performed by chromatography on silica thin layers and they were detected by low temperature (--196 degrees) fluorescence. The parent compounds were regenerated on the chromatogram by spraying with 2 N HC1 and these were detected by low temperature fluorescence. Mercury was also detected in spots by spraying with dithizone. The method provides a simple solution to the problem of oxidation of thiol compounds during isolation procedures.
本文描述了一种将硫唑嘌呤和6-巯基嘌呤的硫醇代谢物转化为苯基汞衍生物的方法。这些衍生物通过硅胶薄层色谱法进行分离，并用低温（-196℃）荧光检测。通过喷洒2N盐酸在色谱图上使母体化合物再生，并用低温荧光检测。通过喷洒双硫腙也可在斑点中检测到汞。该方法为解决分离过程中硫醇化合物的氧化问题提供了一种简单的解决方案。
