采用气相色谱 - 质谱碎片分析法对血浆和尿液中的利多卡因及其脱乙基代谢物进行定量分析。

Quantitation of lidocaine and its deethylated metabolites in plasma and urine by gas chromatography-mass fragmentography.

作者信息

Hignite C E, Tschanz C, Steiner J, Huffman D H, Azarnoff D L

出版信息

J Chromatogr. 1978 Nov 21;161:243-9. doi: 10.1016/s0021-9673(01)85233-x.

Abstract

A sensitive, precise and accurate method for simultaneous quantitation of lidocaine and its deethylated metabolites by gas chromatography-mass fragmentography has been developed. Propyl derivatives of the deethylated metabolites are formed directly in either plasma or urine by treatment with propionaldehyde and sodium cyanoborohydride. The propyl derivatives and unchanged lidocaine are extracted, separated by gas chromatography and quantitated by mass fragmentography using mepivacaine as the internal standard. Quantitation of these compounds to levels as low as 50 ng/ml body fluid has been achieved with coefficients of variation less than 10%.

摘要

已开发出一种灵敏、精确且准确的气相色谱 - 质谱碎片分析法，用于同时定量利多卡因及其脱乙基代谢物。通过用丙醛和氰基硼氢化钠处理，脱乙基代谢物的丙基衍生物可直接在血浆或尿液中形成。丙基衍生物和未变化的利多卡因经提取后，通过气相色谱分离，并用甲哌卡因作为内标，通过质谱碎片分析法进行定量。已实现对这些化合物低至50 ng/ml体液水平的定量，变异系数小于10%。