Hozumi T
J Biochem. 1981 Sep;90(3):785-8. doi: 10.1093/oxfordjournals.jbchem.a133534.
Limited tryptic digestion of the heavy chain of chymotryptic myosin subfragment-1 gives three major fragments with approximate molecular weights of 50 K, 27 K, and 20 K daltons. We previously reported (Hozumi, T. & Muhlrad, A. (1981) Biochemistry 20, 2945-2950) that these fragments can be separated by gel filtration in 1% sodium dodecyl sulfate. By using this method, the tryptophan content and amino acid composition of fragments were measured. The 27 K and 50 K fragments were found to be rich in tryptophan, in contrast to the 20 K fragment.
对胰凝乳蛋白酶肌球蛋白亚片段-1重链进行有限的胰蛋白酶消化可产生三个主要片段，其近似分子量分别为50K、27K和20K道尔顿。我们之前报道过（细见，T. 及米尔拉德，A.（1981年）《生物化学》20，2945 - 2950），这些片段可在1%十二烷基硫酸钠中通过凝胶过滤分离。通过使用此方法，对片段的色氨酸含量和氨基酸组成进行了测定。与20K片段相比，发现27K和50K片段富含色氨酸。
