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Cell-free synthesis of ornithine aminotransferase of rat liver.

作者信息

Hayashi H, Katunuma N, Chiku K, Endo Y, Natori Y

出版信息

J Biochem. 1981 Oct;90(4):1229-32. doi: 10.1093/oxfordjournals.jbchem.a133577.

Abstract

Mitochondrial ornithine aminotransferase was synthesized in a rabbit reticulocyte cell-free system using polysomal mRNA from rat liver. Upon analyzing the primary translation product by electrophoresis and fluorography, we found that ornithine aminotransferase is synthesized not as a large precursor but as one with its authentic size. Free ribosomes were found to be the main site of synthesis of ornithine aminotransferase.

摘要

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Cell-free synthesis of ornithine aminotransferase of rat liver.
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