Richards W D
J Clin Microbiol. 1981 Nov;14(5):587-8. doi: 10.1128/jcm.14.5.587-588.1981.
Alternative methods of storing bovine fecal specimens for delayed culture of Mycobacterium paratuberculosis was studied. Significant (P less than 0.05) losses of viability were sustained by storage at 23 degrees C for 10 days and by treatment with 0.03 Zephiran for 4 and 10 days. Losses were moderate after storage at -70 degrees C for 15 weeks but significant (P greater than 0.05) after storage at 23 degrees C for 4 days. The alternative methods were compared with the standard method of processing specimens upon receipt and after 2 days in transit. Fecal specimens from 18 cattle were cultured.
研究了用于副结核分枝杆菌延迟培养的牛粪便标本的替代储存方法。在23摄氏度下储存10天以及用0.03%的新洁尔灭处理4天和10天,会导致活力显著(P小于0.05)丧失。在-70摄氏度下储存15周后活力丧失程度适中,但在23摄氏度下储存4天后则显著(P大于0.05)。将这些替代方法与收到标本时以及运输2天后处理标本的标准方法进行了比较。对18头牛的粪便标本进行了培养。