Bjerrum O J, Hawkins M, Swanson P, Griffin M, Lorand L
J Supramol Struct Cell Biochem. 1981;16(3):289-301. doi: 10.1002/jsscb.1981.380160309.
An increase in the intracellular concentration of Ca2+ in human erythrocytes results in the formation of gamma-glutamyl-epsilon-lysine cross-linked membrane protein polymers. Following solubilization of the membranes with SDS, these polymers can be isolated on a Lubrol-containing sucrose gradient. Immunoelectrophoresis of the polymeric material with a polyspecific rabbit antibody against human ghosts gave rise to a single, but heterogeneous, precipitate. The polymer was amphiphilic and, on addition to Triton-solubilized erythrocyte membrane proteins, it coprecipitated with spectrin. When the antihost antibody was absorbed with the polymer prior to cross immunoelectrophoresis of normal erythrocyte membrane proteins, the precipitates of glycophorin, acetylcholinesterase, and hemoglobin were normal, whereas the antibody titers against band 3 protein, spectrin, and ankyrin became reduced. Furthermore, a rabbit antibody raised against the isolated human polymer reacted selectively with the same three membrane proteins. No reactions occurred with lysate proteins.
人红细胞内Ca2+浓度的升高会导致γ-谷氨酰-ε-赖氨酸交联的膜蛋白聚合物形成。用SDS溶解膜后，这些聚合物可在含Lubrol的蔗糖梯度上分离。用抗人血影的多特异性兔抗体对聚合材料进行免疫电泳，产生单一但不均一的沉淀。该聚合物具有两亲性，加入到经Triton溶解的红细胞膜蛋白中时，它会与血影蛋白共沉淀。当在正常红细胞膜蛋白的交叉免疫电泳之前用该聚合物吸收抗血影抗体时，血型糖蛋白、乙酰胆碱酯酶和血红蛋白的沉淀正常，而针对带3蛋白、血影蛋白和锚蛋白的抗体效价降低。此外，针对分离出的人聚合物产生的兔抗体与相同的三种膜蛋白发生选择性反应。与裂解物蛋白无反应。
