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双核小体和寡核小体的结构组织。电二色性和双折射研究。

The structural organization of dinucleosomes and oligonucleosomes. Electric dichroism and birefringence study.

作者信息

Houssier C, Lasters I, Muyldermans S, Wyns L

出版信息

Nucleic Acids Res. 1981 Nov 11;9(21):5763-84. doi: 10.1093/nar/9.21.5763.

DOI:10.1093/nar/9.21.5763
PMID:7312629
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327559/
Abstract

The spatial organization of nucleosomes and linker DNA in dinucleosomes and oligonucleosomes of various chain lengths has been investigated through electric dichroism, birefringence and relaxation times measurements at low ionic strengths (0.5 to 2.2 mM). From the negative dichroism observed for all the samples, it is concluded that the nucleosome subunits in the oligonucleosome chain must lie with their disc planes closely parallel to the fibre axis. The large increase of the negative dichroism of dinucleosomes upon Hl removal is interpreted by the unwinding of the DNA tails and the internucleosomal segment. All the samples displayed, under bipolar pulses, a predominantly induced orientation mechanism.

摘要

通过在低离子强度（0.5至2.2 mM）下进行电二色性、双折射和弛豫时间测量，研究了不同链长的双核小体和寡核小体中核小体和连接DNA的空间组织。从所有样品观察到的负二色性得出结论，寡核小体链中的核小体亚基其盘状平面必须与纤维轴紧密平行。去除H1后双核小体负二色性的大幅增加可通过DNA尾部和核小体间片段的解旋来解释。在双极脉冲下，所有样品均表现出主要为诱导取向机制。

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