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1
Nucleosomes arrangement in chromatin.染色质中的核小体排列
Nucleic Acids Res. 1978 Nov;5(11):4431-49. doi: 10.1093/nar/5.11.4431.
2
The structural organization of oligonucleosomes.寡核小体的结构组织
J Biomol Struct Dyn. 1984 Oct;2(2):303-17. doi: 10.1080/07391102.1984.10507569.
3
Higher order structure of chromatin: influence of ionic strength and proteolytic digestion on the birefringence properties of polynucleosomal fibers.染色质的高级结构：离子强度和蛋白酶消化对多核小体纤维双折射特性的影响。
J Biomol Struct Dyn. 1985 Feb;2(4):805-19. doi: 10.1080/07391102.1985.10506325.
4
Linear contour length dependence of electrical polarizability of nucleosomal DNA fragments: implications for the flexibility of DNA.核小体DNA片段电极化率的线性轮廓长度依赖性：对DNA柔韧性的影响
Biochem Biophys Res Commun. 1990 Feb 14;166(3):1140-5. doi: 10.1016/0006-291x(90)90985-v.
5
Histone phosphorylation in native chromatin induces local structural changes as probed by electric birefringence.天然染色质中的组蛋白磷酸化会诱导局部结构变化，这可通过电双折射进行探测。
J Mol Biol. 1985 Nov 20;186(2):367-79. doi: 10.1016/0022-2836(85)90111-1.
6
Structural analysis of Trypanosoma brucei brucei chromatin by limited proteolysis. An electrical-birefringence study.布氏布氏锥虫染色质的有限蛋白酶解结构分析。一项电双折射研究。
Eur J Biochem. 1993 Sep 1;216(2):387-94. doi: 10.1111/j.1432-1033.1993.tb18156.x.
7
Differences in the condensation of chromatin by individual subfractions of histone H1: implications for the role of H1(0) in the structural organization of chromatin.组蛋白H1各亚组分在染色质凝聚方面的差异：对H1(0)在染色质结构组织中作用的启示。
Biochemistry. 1985 Nov 5;24(23):6328-35. doi: 10.1021/bi00344a002.
8
Electron microscopic and biochemical evidence that chromatin structure is a repeating unit.电子显微镜和生化证据表明染色质结构是一个重复单元。
Cell. 1975 Apr;4(4):281-300. doi: 10.1016/0092-8674(75)90149-x.
9
The structural organization of dinucleosomes and oligonucleosomes. Electric dichroism and birefringence study.双核小体和寡核小体的结构组织。电二色性和双折射研究。
Nucleic Acids Res. 1981 Nov 11;9(21):5763-84. doi: 10.1093/nar/9.21.5763.
10
Influence of histone H1 on chromatin structure.组蛋白H1对染色质结构的影响。
Biochem Biophys Res Commun. 1980 May 30;94(2):535-41. doi: 10.1016/0006-291x(80)91264-4.

引用本文的文献

1
Global histone protein surface accessibility in yeast indicates a uniformly loosely packed genome with canonical nucleosomes.酵母中全局组蛋白表面可及性表明其基因组具有典型核小体，呈均匀的松散包装。
Epigenetics Chromatin. 2021 Jan 11;14(1):5. doi: 10.1186/s13072-020-00381-5.
2
The structural organization of dinucleosomes and oligonucleosomes. Electric dichroism and birefringence study.双核小体和寡核小体的结构组织。电二色性和双折射研究。
Nucleic Acids Res. 1981 Nov 11;9(21):5763-84. doi: 10.1093/nar/9.21.5763.
3
1H NMR investigation of the conformational states of DNA in nucleosome core particles.核磁共振氢谱对核小体核心颗粒中DNA构象状态的研究。
Nucleic Acids Res. 1979;6(6):2327-37. doi: 10.1093/nar/6.6.2327.

本文引用的文献

1
Preparation of native chromatin and damage caused by shearing.制备天然染色质和剪切引起的损伤。
Science. 1975 Mar 28;187(4182):1203-6. doi: 10.1126/science.187.4182.1203.
2
New method for studying electrical orientation and relaxation effects in aqueous colloids; preliminary results with tobacco mosaic virus.研究水性胶体中电取向和弛豫效应的新方法；烟草花叶病毒的初步结果。
Science. 1950 Feb;111(2875):113-6. doi: 10.1126/science.111.2875.113.
3
High resolution acrylamide gel electrophoresis of histones.组蛋白的高分辨率丙烯酰胺凝胶电泳
Arch Biochem Biophys. 1969 Mar;130(1):337-46. doi: 10.1016/0003-9861(69)90042-3.
4
The linear dichroism of oriented helical and superhelical polymers.取向螺旋和超螺旋聚合物的线性二色性。
Biopolymers. 1972;11(9):1929-41. doi: 10.1002/bip.1972.360110913.
5
Electric dichroism of chromatin.染色质的电二色性
J Mol Biol. 1974 Mar 15;83(4):459-71. doi: 10.1016/0022-2836(74)90507-5.
6
Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.染色质亚结构。一种核脱氧核糖核酸酶对染色质DNA在规则间隔位点的消化作用。
Biochem Biophys Res Commun. 1973 May 15;52(2):504-10. doi: 10.1016/0006-291x(73)90740-7.
7
Electron microscopy of chromatin subunit particles.染色质亚基颗粒的电子显微镜检查。
Biochem Biophys Res Commun. 1974 Oct 23;60(4):1365-70. doi: 10.1016/0006-291x(74)90348-9.
8
Spheroid chromatin units (v bodies).球状染色质单位（V小体）。
Science. 1974 Jan 25;183(4122):330-2. doi: 10.1126/science.183.4122.330.
9
Studies on chromatin. V. A model for the structure of chromatin subunit.染色质研究。V. 染色质亚基结构模型
Mol Biol Rep. 1975 Oct;2(3):255-62. doi: 10.1007/BF00356996.
10
Thermal denaturation of subchromosomal particles.亚染色体颗粒的热变性
Biochem Biophys Res Commun. 1975 Sep 2;66(1):403-10. doi: 10.1016/s0006-291x(75)80342-1.

染色质中的核小体排列

Nucleosomes arrangement in chromatin.

作者信息

Marion C, Roux B

出版信息

Nucleic Acids Res. 1978 Nov;5(11):4431-49. doi: 10.1093/nar/5.11.4431.

DOI:10.1093/nar/5.11.4431
PMID:724513
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342760/
Abstract

The spatial arrangement of nucleosomes in rat liver chromatin has been examined using the electric birefringence technique. All chromatin subunits studied (up to 9 consecutive nucleosomes) contain their full complement of the five histone types associated with about 200 base pairs repeat length DNA. From the relaxation times and the orientation mechanisms, the nucleosome may be assimilated to an oblate ellipsoid of dimensions about 140 x 140 x 70 A, and the DNA superhelical axis is parallel to its shorter axis. The most important result is a sharp transition in the electro-optical properties of subunits when the number of nucleosomes in the chain is greater than 6 : the initial negative birefringence, as for DNA, becomes positive and the relaxation time is multiplied by ten. The hexanucleosome, which presents no birefringence, has an helical symmetrical structure without preferential orientation axis. This structure is approximatively spherical of about 250 A diameter and the chromatin appears as a periodic array of such a structure.

摘要

利用电双折射技术研究了大鼠肝脏染色质中核小体的空间排列。所有研究的染色质亚基（多达9个连续的核小体）都包含与约200个碱基对重复长度的DNA相关的五种组蛋白的完整互补物。根据弛豫时间和取向机制，核小体可被视为尺寸约为140×140×70埃的扁椭球体，DNA超螺旋轴与其短轴平行。最重要的结果是，当链中核小体的数量大于6时，亚基的电光性质会发生急剧转变：与DNA一样，最初的负双折射变为正双折射，弛豫时间乘以10。没有双折射的六核小体具有螺旋对称结构，没有优先取向轴。这种结构近似为直径约250埃的球形，染色质表现为这种结构的周期性阵列。