Blank A, Dekker C, Schieven G, Sugiyama R, Thelen M
Nucleic Acids Symp Ser. 1981(10):203-9.
Study of the RNases of human body fluids has been facilitated by use of activity staining following SDS-polyacrylamide gel electrophoresis. Commercial SDS preparations contain minor lipophilic contaminants (less than 0.1%) which interfere with enzyme renaturation and prevent activity staining unless gels are washed after electrophoresis in 25% isopropanol. Partial characterization of the RNases of serum, urine, and cerebrospinal fluid (CSF) is described, including evidence that the RNases comprising bands A-C of urine and 1-3 of CSF are glycoproteins. Evidence is presented that the major RNase activities of serum (RNases 1-5) and urine (band A) do not originate in pancreas, and that leukocytes are the source of band D RNase of urine, as well as of minor RNase activities of serum and CSF. Results are summarized suggesting that elevated plasma RNase levels may be of dubious utility in the diagnosis of most malignant diseases. Some elevated levels reported in the literature may reflect the advanced age of cancer patients, negative nitrogen balance, and other secondary effects of diseases, particularly kidney dysfunction.
通过在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）后进行活性染色，人体体液中核糖核酸酶（RNase）的研究得到了促进。市售的SDS制剂含有少量亲脂性污染物（低于0.1%），这些污染物会干扰酶的复性并阻止活性染色，除非在电泳后用25%异丙醇洗涤凝胶。本文描述了血清、尿液和脑脊液（CSF）中RNase的部分特性，包括尿液中A - C条带和脑脊液中1 - 3条带的RNase是糖蛋白的证据。有证据表明，血清（RNase 1 - 5）和尿液（A条带）中的主要RNase活性并非源自胰腺，白细胞是尿液中D条带RNase以及血清和脑脊液中次要RNase活性的来源。研究结果总结表明，血浆RNase水平升高在大多数恶性疾病的诊断中可能用处不大。文献中报道的一些升高水平可能反映了癌症患者的高龄、负氮平衡以及疾病的其他继发效应，尤其是肾功能不全。
