Trout J J, Stauber W T, Schottelius B A
Tissue Cell. 1981;13(2):393-401. doi: 10.1016/0040-8166(81)90013-6.
Ultrastructural and cytochemical techniques were used to investigate autophagy in the tonic anterior(ALD) and phasic posterior (PLD) latissimus dorsi muscles of the chicken following chloroquine administration. Autophagic vacuoles were seen in the ALD after 1 day of chloroquine administration while no change was seen in the PLD until 3 days. In both muscles, autophagic vacuoles and myeloid bodies were found at the level of the I band. Myeloid bodies usually were found in the longitudinal rows of mitochondria in the ALD muscle. Some, but not all, of the autophagic vacuoles and myeloid bodies were cytochemically acid phosphatase positive, while the portion of the sarcoplasmic reticulum of both muscles which is normally acid phosphatase positive, while the portion of the sarcoplasmic reticulum of both muscles which is normally, acid phosphatase positive was devoid of activity following chloroquine administration. These observations are discussed in regard to accepted mechanisms of autophagy and the possible inhibition of autophagy in skeletal muscle tissue by chloroquine.
采用超微结构和细胞化学技术研究了氯喹给药后鸡的强直性前背阔肌（ALD）和相位性后背阔肌（PLD）中的自噬情况。氯喹给药1天后在ALD中可见自噬泡，而PLD直到3天后才出现变化。在两块肌肉中，均在I带水平发现了自噬泡和髓样体。髓样体通常见于ALD肌肉中线粒体的纵行排列中。部分（而非全部）自噬泡和髓样体细胞化学酸性磷酸酶呈阳性，而两块肌肉中通常酸性磷酸酶呈阳性的肌浆网部分，在氯喹给药后失去了活性。结合公认的自噬机制以及氯喹可能对骨骼肌组织自噬的抑制作用对这些观察结果进行了讨论。
