Aldrich H C, Pendland J C
Tissue Cell. 1981;13(2):431-9. doi: 10.1016/0040-8166(81)90016-1.
Freeze-fracture and thin sectioning techniques were used to follow in large synchronous plasmodia of Physarum polycephalum the changes in number and distribution of nuclear pores during the cell cycle. Using freeze-fracture, we determined that average pore frequency rises gradually from 14/micrometers(2) of nuclear envelope surface at early S to a value of about 22 just before prophase. Nuclear diameter averaged 3.3 micrometers at early S and increased to 4.3 micrometers at late G2. Calculating nuclear volume and average chromatin volume per nucleus with respect to time in the cell cycle leads to the conclusion that number of nuclear pores appears to be most directly related to amount of chromatin present per nucleus and to be independent of nuclear surface area.
采用冷冻蚀刻和超薄切片技术，追踪多头绒泡菌大量同步化原质团在细胞周期中核孔数量和分布的变化。通过冷冻蚀刻，我们确定平均孔频率从S期早期核膜表面的14个/μm²逐渐上升，到前期前达到约22个/μm²。S期早期核直径平均为3.3μm，G2期末增加到4.3μm。计算细胞周期中不同时间点的核体积和每个核的平均染色质体积，得出结论：核孔数量似乎与每个核中存在的染色质数量最直接相关，且与核表面积无关。
