Herdugo G G, Tyson J J
Exp Cell Res. 1984 Aug;153(2):570-3. doi: 10.1016/0014-4827(84)90627-x.
Macroplasmodia of the acellular slime mold Physarum polycephalum were treated with pulses of cycloheximide (10 micrograms/ml medium, for 3 h), initiated 10-20 min before metaphase in the synchronous nuclear division cycle. This treatment interfered with normal division of the nuclei, but permitted DNA synthesis in the next S phase. This interpretation is supported by measurements of the DNA content per nucleus in cycloheximide-treated cultures as compared to control cultures, which show that some nuclei after cycloheximide treatment are polyploid. By this method we can produce polyploid strains of Physarum, but the elevated nuclear DNA content is not stable, and after several months the strains have reverted to the normal diploid DNA content.
在多核黏菌多头绒泡菌的同步核分裂周期中，在中期前10 - 20分钟开始，用环己酰亚胺脉冲处理（10微克/毫升培养基，处理3小时）。这种处理干扰了细胞核的正常分裂，但允许在下一个S期进行DNA合成。与对照培养物相比，对经环己酰亚胺处理的培养物中每个细胞核的DNA含量进行测量，支持了这一解释，测量结果表明，经环己酰亚胺处理后，一些细胞核是多倍体。通过这种方法，我们可以产生多头绒泡菌的多倍体菌株，但细胞核DNA含量的升高并不稳定，几个月后这些菌株又恢复到正常的二倍体DNA含量。
