一种通过在硅油混合物上进行等密度差速离心来富集网织红细胞的简单方法。
A simple procedure for the enrichment of reticulocytes by isodense differential centrifugation on silicone oil mixtures.
作者信息
Gellerich F N, Spengler V, Augustin W
出版信息
Acta Biol Med Ger. 1981;40(4-5):611-5.
Utilizing age-dependent differences in the density of red cells, a mixture of silicone oils with density of 1.0806 g/ml was used for the enrichment of rabbit reticulocytes. During concentration, heavy cells, mainly consisting of erythrocytes, move into the silicone oil, whereas the reticulocytes remain above the oil. Suspensions of apparently intact cells with reticulocyte counts of 87 +/- 7.8% and a recovery rate of about 37% were reproducibly obtained. Neither inhibition of cell respiration nor depletion of adenine nucleotides were observed after this enrichment procedure. A micro-modification in hematocrit capillaries seems to be applicable to obtain rapid information about reticulocyte contents in blood samples without staining and counting of cells.
利用红细胞密度的年龄依赖性差异，使用密度为1.0806 g/ml的硅油混合物来富集兔网织红细胞。在浓缩过程中，主要由红细胞组成的重细胞移入硅油中，而网织红细胞则留在油层上方。可重复获得网织红细胞计数为87±7.8%且回收率约为37%的明显完整细胞悬液。在此富集过程后，未观察到细胞呼吸抑制或腺嘌呤核苷酸耗竭。血细胞比容毛细管的一种微改进似乎适用于在不进行细胞染色和计数的情况下快速获取血样中网织红细胞含量的信息。
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