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脆弱拟杆菌NCTC 9343的沉淀性碳水化合物抗原

Precipitating carbohydrate antigens of Bacteroides fragilis NCTC 9343.

作者信息

Hofstad T

出版信息

Acta Pathol Microbiol Scand B. 1981 Aug;89(4):215-9. doi: 10.1111/j.1699-0463.1981.tb00179_89b.x.

Abstract

An antigen fraction isolated by various forms of chromatography from phenol/water extracts of Bacteroides fragilis NCTC 9343 was devoid of phosphorus, fatty acids and amino acids. Rhamnose, fucose, galactose, glucose and a neutral sugar tentatively identified as arabinose were present at molar ratios of 1:1:1.2:9.0.9:0.7. The fraction contained an unidentified reducing and ninhydrin positive substance and trace amounts of glucosamine and galactosamine. The antigen fraction produced three immunoelectrophoretic bands against NCTC 9343 antiserum, and reacted in complement fixation tests.

摘要

通过各种色谱形式从脆弱拟杆菌NCTC 9343的苯酚/水提取物中分离出的一种抗原组分不含磷、脂肪酸和氨基酸。鼠李糖、岩藻糖、半乳糖、葡萄糖和一种暂定为阿拉伯糖的中性糖的摩尔比为1:1:1.2:9.0:0.9:0.7。该组分含有一种未鉴定的还原且茚三酮阳性物质以及痕量的氨基葡萄糖和半乳糖胺。该抗原组分针对NCTC 9343抗血清产生了三条免疫电泳带,并在补体结合试验中发生反应。

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