C3b受体在人肾小球中的定位。

Localization of the C3b receptors in human renal glomeruli.

作者信息

Matre R, Nilsen R, Iversen B M

出版信息

Acta Pathol Microbiol Scand C. 1981 Oct;89(5):311-3. doi: 10.1111/j.1699-0463.1981.tb02705.x.

DOI:10.1111/j.1699-0463.1981.tb02705.x

Abstract

Cryostat sections of human kidney were incubated with C3b containing immune complexes. The complexes were horseradish peroxidase (HRP) and F(ab')2 fragments of rabbit IgG anti-HRP incubated in C4-deficient guinea pig serum. The peroxidase activity was revealed histochemically. The sections were studied using light and electron microscopy. The bound complexes were localized to the foot processes of the visceral epithelial cells and to the surface of the parietal epithelial cells facing Bowman's space. No receptors could be demonstrated on the endothelial or mesangial cells.

摘要

将人肾的低温恒温器切片与含C3b的免疫复合物一起孵育。这些复合物是辣根过氧化物酶（HRP）和兔抗HRP IgG的F(ab')2片段，在缺乏C4的豚鼠血清中孵育。通过组织化学方法显示过氧化物酶活性。使用光学显微镜和电子显微镜对切片进行研究。结合的复合物定位于脏层上皮细胞的足突以及面对鲍曼间隙的壁层上皮细胞表面。在内皮细胞或系膜细胞上未发现受体。