O'Rear E A, Udden M M, McIntire L V, Lynch E C
Am J Hematol. 1981 Nov;11(3):283-92. doi: 10.1002/ajh.2830110309.
As calcium has increasingly been the object of study in erythrocyte physiology, we reviewed the current methodologies for determination of calcium by atomic absorption spectrometry. The published normal values for erythrocyte calcium vary from 5 to 500 mumol/liter of packed cells. A method based on Harrison and Long's determination of calcium is presented and shows normal red cell calcium concentration to be 0.0149 +/- 0.0023 mumol/ml of packed red cells. The influence of temperature and type of crucible used in ashing red cells is assessed. The method of additions is employed to corroborate our results.
由于钙越来越成为红细胞生理学研究的对象，我们综述了目前用原子吸收光谱法测定钙的方法。已发表的红细胞钙的正常值在每升压实细胞5至500微摩尔之间。本文介绍了一种基于哈里森和朗的钙测定方法，该方法显示正常红细胞钙浓度为每毫升压实红细胞0.0149±0.0023微摩尔。评估了灰化红细胞时温度和坩埚类型的影响。采用加样法来证实我们的结果。
