Gettins P, Dwek R A, Perutz R N
Biochem J. 1981 Jul 1;197(1):119-25. doi: 10.1042/bj1970119.
Pre-resonance Raman spectroscopy was used to study the interactions of the nitro groups of dinitrophenyl haptens with three dinitrophenyl-binding antibody fragments: M315 Fv, M460 Fab' and X25 Fab'. The observed changes in frequency of modes associated with the nitro moieties are compared with solvent-induced changes for the model hapten 2,4-dinitroaniline. These comparisons demonstrate a specific interaction via the H2N--C--C--2-NO2 and 4-NO2 groups with the protein. The interaction with the 4-NO2 group appears to be absent for epsilon-N-2,4-dinitrophenyl-L-lysine bound to M315 Fv fragment in contrast with either 2,4-dinitrophenylaspartate or 2,4-dinitrophenylglycine bound to M315 Fv fragment, despite the much tighter binding of the lysine derivative. The implications of this for M315 Fv fragment in terms of the antibody specificity are discussed. Comparisons of the effect of binding to M460 Fab' and X25 Fab' fragments also revealed significant differences in the shifts of the nitro group vibrations of 2,4-dinitrophenyl-lysine and 2,4-dinitroaniline.
M315 Fv、M460 Fab' 和 X25 Fab' 的相互作用。将观察到的与硝基部分相关的模式频率变化与模型半抗原2,4-二硝基苯胺的溶剂诱导变化进行比较。这些比较表明通过H2N--C--C--2-NO2和4-NO2基团与蛋白质存在特异性相互作用。与2,4-二硝基苯天冬氨酸或2,4-二硝基苯甘氨酸结合到M315 Fv片段相比，与M315 Fv片段结合的ε-N-2,4-二硝基苯基-L-赖氨酸似乎不存在与4-NO2基团的相互作用，尽管赖氨酸衍生物的结合更紧密。讨论了这对M315 Fv片段抗体特异性的影响。与M460 Fab' 和X25 Fab' 片段结合效果的比较也揭示了2,4-二硝基苯基赖氨酸和2,4-二硝基苯胺的硝基基团振动位移存在显著差异。
