Husson A, Fairand A, Vaillant R
Biol Neonate. 1981;40(5-6):224-31. doi: 10.1159/000241496.
Urea synthesis in fetal rats was studied using a liver slice system with ammonium chloride as nitrogen source. In term fetuses, the rate of urea formation increases with ammonium chloride concentration up to 100 microM and is enhanced by the addition of ornithine. The developmental pattern for urea synthesis is very similar to that found in the enzyme activity studies: urea production increases slightly during fetal life and then rises rapidly at birth. It is found that fetal liver has an absolute requirement for glucocorticosteroids to develop a normal urea synthesis. The accordance with argininosuccinate synthetase activity measured in liver homogenate is discussed.
利用以氯化铵为氮源的肝切片系统，对胎鼠的尿素合成进行了研究。在足月胎儿中，尿素生成速率随氯化铵浓度增加至100微摩尔而升高，并因添加鸟氨酸而增强。尿素合成的发育模式与酶活性研究中发现的模式非常相似：胎儿期尿素生成略有增加，然后在出生时迅速上升。发现胎儿肝脏对糖皮质激素有绝对需求，以发展正常的尿素合成。文中讨论了与肝匀浆中测得的精氨琥珀酸合成酶活性的一致性。
