Goldberg D M, Roomi M W, Yu A, Roncari D A
Can J Biochem. 1981 Sep;59(9):793-8. doi: 10.1139/o81-109.
Fasting male rats fed 3-methylcholanthrene in a daily dose of 40 mg . kg body weight-1 gave evidence of hepatic microsomal enzyme induction after 3 days through significantly increased hepatic aryl hydrocarbon hydroxylase activity, cytochrome P448 content, and characteristic changes in microsomal proteins analysed by sodium dodecyl sulfate--polyacrylamide gel electrophoresis. Concomitantly, activities of aminopyrine N-demethylase and microsomal gamma-glutamyltransferase, which are increased in phenobarbital-treated rats, significantly declined. In contrast to phenobarbital, which has been previously shown to increase hepatic triacylglycerol content and in vitro glycerolipid synthesis, 3-methylcholanthrene did not affect hepatic triacylglycerol content, but did inhibit glycerolipid synthesis by cell-free preparations of rat liver and significantly reduced serum triacylglycerol concentration. Thus, the two prototypical drugs inducing characteristically different changes in microsomal enzyme and hemoprotein response also seem to differ in their effect upon another important microsomal function, hepatic glycerolipid synthesis.
给禁食的雄性大鼠每日按40毫克/千克体重的剂量喂食3-甲基胆蒽，3天后，通过肝芳烃羟化酶活性显著增加、细胞色素P448含量增加以及用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析的微粒体蛋白特征性变化，证明了肝微粒体酶的诱导作用。同时，在苯巴比妥处理的大鼠中增加的氨基比林N-脱甲基酶和微粒体γ-谷氨酰转移酶的活性显著下降。与先前已证明会增加肝三酰甘油含量和体外甘油脂质合成的苯巴比妥不同，3-甲基胆蒽不影响肝三酰甘油含量，但确实抑制大鼠肝脏无细胞制剂的甘油脂质合成，并显著降低血清三酰甘油浓度。因此，这两种在微粒体酶和血红蛋白反应中诱导特征性不同变化的典型药物，在对另一个重要的微粒体功能即肝甘油脂质合成的影响上似乎也有所不同。
