Belisario M A, Borgia R, Pecce R, de Lorenzo F
Istituto di Biochimica Cellulare e Molecolare, II Facoltà di Medicina e Chirurgia, Napoli, Italy.
Environ Res. 1988 Feb;45(1):91-100. doi: 10.1016/s0013-9351(88)80011-2.
The inducing effects of 1-nitropyrene (1-NP) on the microsomal cytochrome P-450 system were studied in rats. Intraperitoneal administration of 1-NP led to increases in cytochrome P-450 content and aryl hydrocarbon hydroxylase, ethoxycoumarin, and ethoxyresorufin-O-deethylase activities. These increases were dose dependent. Cytochrome b5 content and aminopyrine and p-nitroanisole demethylase activities were not affected by treatment of rats with 1-NP. Substrate specificity, sensitivity to mixed-function oxidase inhibitors, and electrophoretic pattern of 1-NP-induced cytochrome(s) P-450 were compared to the major forms of cytochrome P-450 induced by phenobarbital and methylcholanthrene. Furthermore microsomes from 1-NP-induced rats showed greater ability to metabolize the chemical as compared with those from control animals; this result indicates that 1-NP induces a form(s) of cytochrome P-450 especially effective in the metabolism of the substance itself.
研究了1-硝基芘（1-NP）对大鼠微粒体细胞色素P-450系统的诱导作用。腹腔注射1-NP导致细胞色素P-450含量以及芳烃羟化酶、乙氧香豆素和乙氧试卤灵-O-脱乙基酶活性增加。这些增加呈剂量依赖性。细胞色素b5含量以及氨基比林和对硝基苯甲醚脱甲基酶活性不受1-NP处理大鼠的影响。将1-NP诱导的细胞色素P-450的底物特异性、对混合功能氧化酶抑制剂的敏感性和电泳图谱与苯巴比妥和甲基胆蒽诱导的细胞色素P-450的主要形式进行了比较。此外，与对照动物的微粒体相比，1-NP诱导大鼠的微粒体显示出更强的化学物质代谢能力；该结果表明，1-NP诱导了一种对该物质本身代谢特别有效的细胞色素P-450形式。
