遗传性高血压大鼠和去氧皮质酮-盐性高血压大鼠脑和脊髓中的肾上腺素能神经元及苯乙醇胺氮位甲基转移酶活性

Adrenaline neurons and PNMT activity in the brain and spinal cord of genetically hypertensive rats and rats with DOCA--salt hypertension.

Chalmers J P, Howe P R, Wallmann Y, Tumuls I

Clin Sci (Lond). 1981 Dec;61 Suppl 7:219s-221s. doi: 10.1042/cs061219s.

Abstract

We have studied the number of phenylethanolamine-N-methyltransferase (PNMT)-containing nerve cells in the medulla and the activity of PNMT in the medulla, spinal cord and hypothalamus of the rat. 2. At 4 weeks of age there was an increase in the number of PNMT cells counted in the medulla of the spontaneously hypertensive rat (SHR; 21%, P less than 0.01) and the stroke-prone spontaneously hypertensive rat (SHR-SP; 22%, P less than 0.01) compared with the Wistar--Kyoto (WKY) control rat. 3. At 4 months of age there were no significant differences in the number of medullary PNMT cells in two normotensive strains (WKY and Fisher rats), two genetically hypertensive strains (SHR and SHR-SP) and in DOCA--salt hypertensive rats. 4. In four week old rats the activity of PNMT was increased by about 50% in the spinal cord and medulla of the SHR and SHR-SP compared with the WKY rats, and immunotitration experiments suggest that this is due to an increased concentration of enzyme. 5. At 4 months of age there were no increases in PNMT activity of either genetically hypertensive rats or DOCA--salt hypertensive rats.

摘要

我们研究了大鼠延髓中含苯乙醇胺-N-甲基转移酶（PNMT）的神经细胞数量以及延髓、脊髓和下丘脑的PNMT活性。2. 4周龄时，与Wistar-Kyoto（WKY）对照大鼠相比，自发性高血压大鼠（SHR；增加21%，P<0.01）和易卒中型自发性高血压大鼠（SHR-SP；增加22%，P<0.01）延髓中计数的PNMT细胞数量增加。3. 4月龄时，两种正常血压品系（WKY和Fisher大鼠）、两种遗传性高血压品系（SHR和SHR-SP）以及DOCA-盐性高血压大鼠的延髓PNMT细胞数量无显著差异。4. 4周龄大鼠中，与WKY大鼠相比，SHR和SHR-SP的脊髓和延髓中PNMT活性增加约50%，免疫滴定实验表明这是由于酶浓度增加所致。5. 4月龄时，遗传性高血压大鼠或DOCA-盐性高血压大鼠的PNMT活性均未增加。