某些哺乳动物垂体和胎盘激素对蜥蜴睾丸雄激素产生的体外影响。

In vitro influence of some mammalian pituitary and placental hormones on androgen production by the lizard testis.

作者信息

Arslan M, Zaidi A A, Khan S A, Lobo J, Qazi M H

出版信息

Endokrinologie. 1981 Oct;78(1):111-4.

Abstract

Effect of mammalian pituitary and placental hormones has been studied on in vitro androgen production by the testis of the spiny-tailed lizard, Uromastix hardwicki. Aliquots of pooled testicular tissue from adult lizards were incubated individually with 1 microgram each, of oFSH, oLH, oTSH and 1 IU each of hCG, PMSG, and ACTH. Androgen was measured by testosterone radioimmunoassay in both the tissue and the incubation medium. Severalfold increase in androgen production was observed in the testicular tissue incubated with FSH, LH, TSH nd PMSG. Maximum stimulation was obtained with FSH. hCG also caused a measurable increase in androgen production but was much less potent than PMSG. Incubation of testicular tissue with ACTH did not result in a significant change in androgen production as compared to the controls. The hormonal specificity for steroidogenesis by the lizard testis has been discussed in the light of these results.

摘要

研究了哺乳动物垂体和胎盘激素对硬尾蜥（Uromastix hardwicki）睾丸体外雄激素生成的影响。将成年蜥蜴的合并睾丸组织等分试样分别与1微克的卵泡刺激素（oFSH）、促黄体生成素（oLH）、促甲状腺激素（oTSH）以及1国际单位的人绒毛膜促性腺激素（hCG）、孕马血清促性腺激素（PMSG）和促肾上腺皮质激素（ACTH）进行孵育。通过睾酮放射免疫测定法测量组织和孵育培养基中的雄激素。在用FSH、LH、TSH和PMSG孵育的睾丸组织中，观察到雄激素生成增加了数倍。FSH的刺激作用最强。hCG也导致雄激素生成有可测量的增加，但效力远低于PMSG。与对照组相比，用ACTH孵育睾丸组织并未导致雄激素生成发生显著变化。根据这些结果讨论了蜥蜴睾丸类固醇生成的激素特异性。