Condò S G, Giardina B, Lunadei M, Ferracin A, Brunori M
Eur J Biochem. 1981 Nov;120(2):323-7. doi: 10.1111/j.1432-1033.1981.tb05707.x.
The functional properties of hemoglobins from Triturus cristatus carnifex have been characterized both from an equilibrium and a kinetic point of view. A special attention has been given to the effect of organic phosphates in view of their role in the modulation of the hemoglobin function. Under stripped conditions newt hemoglobins display a negative Bohr effect which becomes much less pronounced in the presence of 0.2 M inorganic phosphates. The effect of organic phosphates involves not only a drop in the O2 affinity but also a large change in the shape of the O2 Bohr effect which becomes markedly positive in the presence of myo-inositol hexakis(phosphate). The experimental data reported make it possible to give a complete picture of the functional behaviour of newt hemoglobins, making a contribution to clarifying the dependence of the sign of the Bohr effect on the acclimatization temperature of the animals that has been previously reported [Morpurgo, G., Battaglia, P. A. and Leggio, T. (1970) Nature (Lond.) 225, 76--77].
从平衡和动力学角度对意大利真螈血红素的功能特性进行了表征。鉴于有机磷酸盐在血红蛋白功能调节中的作用，对其影响给予了特别关注。在去除其他物质的条件下，蝾螈血红蛋白呈现负玻尔效应，而在存在0.2M无机磷酸盐时，这种效应变得不那么明显。有机磷酸盐的影响不仅涉及氧气亲和力的下降，还涉及氧气玻尔效应形状的巨大变化，在存在肌醇六磷酸的情况下，这种效应会明显变为正效应。所报道的实验数据使得能够全面了解蝾螈血红蛋白的功能行为，有助于阐明先前报道的玻尔效应符号对动物适应温度的依赖性[莫尔普戈，G.，巴塔利亚，P.A.和莱焦，T.（1970年）《自然》（伦敦）225，76 - 77]。
