Daniels E
Exp Hematol. 1981 Aug;9(7):697-710.
Gelfoam sponge cultures of stromal cells, derived from guinea pig marrow-depleted bone fragments, support the growth of myeloid precursors in the absence of exogenous stimulants. EM radioautographic studies were undertaken to identify active synthesis and distribution of 3H-fucose-containing glycoproteins within the 3-D organization of cultured hemopoietic tissue. Labeling of 5-14 day cultures with tritiated fucose for 20 min revealed an intracellular localization of silver grains over the endoplasmic reticulum and Golgi bodies of all stromal cells. Cumulative labeling (1-48 h) of similar cultures indicated that (i) silver grains subsequently accumulated in plasma membranes, large cytoplasmic granules and also within lipid droplets, (ii) primitive and myeloid cells were also labeled. Localized extracellular concentrations of silver grains were facilitated by the multiple inter-stromal cell contacts and by the accumulation of fine labeled cytoplasmic processes. Mechanisms whereby stromal cells may actively influence physiochemical changes in the hemopoietic inductive microenvironment are suggested.
源自豚鼠骨髓耗竭骨碎片的基质细胞的明胶海绵培养物，在无外源性刺激物的情况下支持髓系前体细胞的生长。进行了电子显微镜放射自显影研究，以确定含3H-岩藻糖糖蛋白在培养造血组织的三维结构中的活性合成和分布。用氚化岩藻糖对5-14天的培养物标记20分钟，显示所有基质细胞的内质网和高尔基体上银颗粒的细胞内定位。对类似培养物的累积标记（1-48小时）表明：（i）银颗粒随后积聚在质膜、大的细胞质颗粒以及脂滴内，（ii）原始细胞和髓系细胞也被标记。基质细胞间的多次接触以及精细标记的细胞质突起的积累促进了银颗粒在细胞外的局部聚集。文中提出了基质细胞可能积极影响造血诱导微环境中物理化学变化的机制。
