Stĕrba J, Slais J, Machnicka B, Schandl V
Folia Parasitol (Praha). 1981;28(4):353-8.
Two experimental bulls (weight 200 kg each) were infected with activated oncospheres of Taenia saginata. The infective dose (160,000 oncospheres) was administered twice to each animal, once by oral, once by subcutaneous routes. At 6 hr p.i., a boil appeared at the site of puncture which attained a diameter of 5.5 cm and 6.2 cm respectively within 3 days, and a thickness of 1.8 cm and 2.2 cm. The postmortem disclosed an extremely weak total infestation of the muscles (approximately 30 cysticeri). The inflammatory tissue of subcutaneous foci contained eggs with an embryophore. Part of the eggs were enclosed in multinucleate giant cells. The tissue reaction differed in dependence on the viability of the oncosphere in the egg. Either giant cells were produced as in the presence of a foreign body, or there was a local accumulation of eosinophiles which after their destruction, were replaced by a crystalline conglomerate containing an egg in its centre. In addition, we found young stages of cysticerci affected by dystrophy. Remarkable eosinophile substances observed on the surface both of some eggs and of developing cysticerci (an analogy of the Splendone-Hoepli phenomenon) apparently indicated a binding of antibodies to the antigenic structure.
选用两头实验公牛（每头体重200千克），用激活的牛肉绦虫六钩蚴进行感染。每头动物均接受两次感染剂量（160,000个六钩蚴），一次经口给药，一次经皮下给药。感染后6小时，穿刺部位出现脓肿，3天内直径分别达到5.5厘米和6.2厘米，厚度达到1.8厘米和2.2厘米。尸检显示肌肉的总体感染程度极轻（约30个囊尾蚴）。皮下病灶的炎性组织含有带胚膜的虫卵。部分虫卵被多核巨细胞包裹。组织反应因虫卵内六钩蚴的活力不同而有所差异。要么像存在异物时那样产生巨细胞，要么出现嗜酸性粒细胞局部聚集，嗜酸性粒细胞破坏后，被中心含有虫卵的结晶聚集体取代。此外，我们发现了受营养不良影响的囊尾蚴幼虫阶段。在一些虫卵和发育中的囊尾蚴表面观察到显著的嗜酸性物质（类似于施彭多内 - 赫普利现象），显然表明抗体与抗原结构发生了结合。
