Nakayama S, Sakashita M, Nishimura T, Sakamoto K
Nihon Yakurigaku Zasshi. 1981 Aug;78(2):91-107.
Experimental hyperlipemia induced by a high cholesterol diet (HCD) in male Sprague-Dawley rats was investigated by measuring lipid levels in serum, liver and aorta. For old rats were fed a laboratory chow diet containing 0.5, 1.0 and 2.0% of cholesterol, for 6 weeks. Serum total lipid (TL) and total cholesterol (TC) in 1.0% HCD were markedly increased and reached peaks by feeding HCD for 18 to 21 days. Although the degree of increase in serum TL and TC was similar in both 0.5 and 2.0% HCD groups, these levels decreased more rapidly in the former and more slowly in the latter, after the levels had been reached a peak. The serum free cholesterol level reached a peak in those fed 0.5% HCD for 12 days and those fed 1.0% HCD for 21 days, but the subsequent reduction was smaller in extent as compared with serum TL and TC. Serum phospholipid (PL) level reached a peak in those fed both 0.5 and 1.0% HCD groups for 12 days and this level was maintained until 42 days in the 1.0% HCD group. Serum triglyceride (TG) levels increased during the first half of the experimental period, but decreased in the second half, with no significant difference between the 0.5 and 1.0% HCD groups. Cholesterol in high density lipoprotein (HCD-C) decreased in rats on the HCD and there was a tendency toward reversion to normal levels from the 4th week in the group on the 2.0% diet, however, a continual decrease occurred in the 0.5 and 1.0% groups. The change in phospholipid in HDL (HDL-PL) was similar to that of HDL-C in both the 0.5 and 1.0% HCD groups. In liver lipids, TL and TC were markedly increased by HCD, but TG increased at first and then decreased as did serum TG. Liver PL decreased by 0.5 and 1.0% HCD groups. In aorta lipids, TL and TC decreased. As a remarkable increase in serum lipids and decrease in HDL-C and HDL-PL were continuous in the 1.0% HCD in comparison with 0.5 or 2.0% HCD, 1.0% HCD appears to be the most suitable experimental model of hyperlipemia in rats. In addition, it is considered that 0.5% HCD is suitable for investigation of the effect of a drug for a relatively short period of treatment.
通过测量雄性斯普拉格-道利大鼠血清、肝脏和主动脉中的脂质水平，研究了高胆固醇饮食（HCD）诱导的实验性高脂血症。将老年大鼠喂食含0.5%、1.0%和2.0%胆固醇的实验室普通饲料，持续6周。1.0% HCD组的血清总脂质（TL）和总胆固醇（TC）显著升高，并在喂食HCD 18至21天时达到峰值。虽然0.5%和2.0% HCD组血清TL和TC的升高程度相似，但在达到峰值后，前者的这些水平下降更快，后者下降更慢。血清游离胆固醇水平在喂食0.5% HCD 12天的大鼠和喂食1.0% HCD 21天的大鼠中达到峰值，但与血清TL和TC相比，随后的降低幅度较小。血清磷脂（PL）水平在喂食0.5%和1.0% HCD组12天时达到峰值，并且在1.0% HCD组中该水平维持到42天。血清甘油三酯（TG）水平在实验期的前半段升高，但在后半段降低，0.5%和1.0% HCD组之间无显著差异。高密度脂蛋白胆固醇（HDL-C）在HCD喂养的大鼠中降低，在2.0%饮食组中从第4周开始有恢复到正常水平的趋势，然而，在0.5%和1.0%组中持续下降。0.5%和1.0% HCD组中高密度脂蛋白磷脂（HDL-PL）的变化与HDL-C相似。在肝脏脂质中，HCD使TL和TC显著升高，但TG起初升高，然后像血清TG一样下降。0.5%和1.0% HCD组肝脏PL降低。在主动脉脂质中，TL和TC降低。与0.5%或2.0% HCD相比，1.0% HCD组血清脂质显著升高以及HDL-C和HDL-PL持续降低，1.0% HCD似乎是大鼠高脂血症最适合的实验模型。此外，认为0.5% HCD适合在相对较短的治疗期内研究药物的效果。
