Kugler P
Histochemistry. 1981;73(1):137-50. doi: 10.1007/BF00493140.
The human endosalpingeal ciliated cells are not a homogeneous cell population. They can be distinguished into mitochondria prominent and normal cells. The morphological appearance of ciliated cells was studied in organ culture using different hormones and hormone combinations in the culture media. Histological (HE, semithin sections) and histochemical methods (PAS, Alcian Blue, SDH, LDH, ATPase, 5'-Nucleotidase, acid and alkaline Phosphatase) were applied. Those mitochondria prominent ciliated cells which are seen in the native endosalpinx can in vitro also be determined mainly under the influence of steroid hormones (hydrocortisone and progesterone). In hormone free incubation media the apices of ciliated cells are separated. This will happen in hormone containing media, too, but with delay, however. Some results are in agreement with the possible transformation from ciliated cells to secretory cells.
人类输卵管内膜纤毛细胞并非单一的细胞群体。它们可分为线粒体突出型细胞和正常细胞。利用培养基中不同的激素及激素组合,在器官培养中对纤毛细胞的形态外观进行了研究。应用了组织学方法(苏木精 - 伊红染色、半薄切片)和组织化学方法(过碘酸 - 希夫反应、阿尔辛蓝染色、琥珀酸脱氢酶、乳酸脱氢酶、三磷酸腺苷酶、5'-核苷酸酶、酸性和碱性磷酸酶)。在天然输卵管内膜中可见的那些线粒体突出型纤毛细胞,在体外主要也可在类固醇激素(氢化可的松和孕酮)的影响下被确定。在无激素的孵育培养基中,纤毛细胞的顶端会分离。在含激素的培养基中也会发生这种情况,但会延迟。一些结果与纤毛细胞可能向分泌细胞转化的情况相符。