Witkowska E
Folia Histochem Cytochem (Krakow). 1979;17(3):239-50.
The effect of certain gonadotropic and steroid hormones on the epithelial cells of the bovine cultured in vitro was investigated. The effect of the hormones on the activity of acid and alkaline phosphatases was observed. Application of hormones to the medium caused an increase of the amount of glycogen in cells. The activity of alkaline phosphatase increased mostly after application of estradiol (0.2 microgram), progesterone (2 microgram) and the luteinizing hormone (0.1 microgram), and the activity of acid phosphatase after the minimum dose of the luteinizing hormone and estradiol, and the maximum dose of progesterone. On the basis of morphological and histochemical observations, it was assumed that the epithelial cells of the bovine oviduct cultured in vitro may undergo transformations.
研究了某些促性腺激素和类固醇激素对体外培养的牛上皮细胞的作用。观察了这些激素对酸性和碱性磷酸酶活性的影响。将激素添加到培养基中会导致细胞内糖原含量增加。碱性磷酸酶的活性在应用雌二醇(0.2微克)、孕酮(2微克)和促黄体生成素(0.1微克)后大多增加,而酸性磷酸酶的活性在促黄体生成素和雌二醇的最小剂量以及孕酮的最大剂量作用后增加。基于形态学和组织化学观察,推测体外培养的牛输卵管上皮细胞可能会发生转化。