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牛输卵管上皮细胞在体外对外源性促性腺激素和类固醇激素的反应性。第二部分:促性腺激素对糖原以及酸性和碱性磷酸酶含量的影响。

Reactivity of the epithelial cells of the bovine oviduct in vitro on the exogenic, gonadotropic and steroid hormones. Part II: The effect of gonadotropic hormones on the amount of glycogen and of acid and alkaline phosphatases.

作者信息

Witkowska E

出版信息

Folia Histochem Cytochem (Krakow). 1979;17(3):239-50.

PMID:488859
Abstract

The effect of certain gonadotropic and steroid hormones on the epithelial cells of the bovine cultured in vitro was investigated. The effect of the hormones on the activity of acid and alkaline phosphatases was observed. Application of hormones to the medium caused an increase of the amount of glycogen in cells. The activity of alkaline phosphatase increased mostly after application of estradiol (0.2 microgram), progesterone (2 microgram) and the luteinizing hormone (0.1 microgram), and the activity of acid phosphatase after the minimum dose of the luteinizing hormone and estradiol, and the maximum dose of progesterone. On the basis of morphological and histochemical observations, it was assumed that the epithelial cells of the bovine oviduct cultured in vitro may undergo transformations.

摘要

研究了某些促性腺激素和类固醇激素对体外培养的牛上皮细胞的作用。观察了这些激素对酸性和碱性磷酸酶活性的影响。将激素添加到培养基中会导致细胞内糖原含量增加。碱性磷酸酶的活性在应用雌二醇(0.2微克)、孕酮(2微克)和促黄体生成素(0.1微克)后大多增加,而酸性磷酸酶的活性在促黄体生成素和雌二醇的最小剂量以及孕酮的最大剂量作用后增加。基于形态学和组织化学观察,推测体外培养的牛输卵管上皮细胞可能会发生转化。

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1
Reactivity of the epithelial cells of the bovine oviduct in vitro on the exogenic, gonadotropic and steroid hormones. Part II: The effect of gonadotropic hormones on the amount of glycogen and of acid and alkaline phosphatases.牛输卵管上皮细胞在体外对外源性促性腺激素和类固醇激素的反应性。第二部分:促性腺激素对糖原以及酸性和碱性磷酸酶含量的影响。
Folia Histochem Cytochem (Krakow). 1979;17(3):239-50.
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Reactivity of the epithelial cells of the bovine oviduct in vitro on the exogenic gonadotropic and steroid hormones. Part I: The effect of gonadotropic and steroid hormones on the amount of lipids and activity of dehydrogenases.牛输卵管上皮细胞在体外对外源性促性腺激素和甾体激素的反应性。第一部分:促性腺激素和甾体激素对脂质含量及脱氢酶活性的影响
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Hoppe Seylers Z Physiol Chem. 1974 Oct;355(10):1259.

引用本文的文献

1
Bovine oviductal epithelial cells: their cell culture and applications in studies for reproductive biology.牛输卵管上皮细胞:它们的细胞培养及其在生殖生物学研究中的应用。
Cytotechnology. 1997 Jan;23(1-3):171-83. doi: 10.1023/A:1007929826186.